Introduction and Objective: Diabetes and adipose research generate rich datasets whose accessibility and integration are often limited. To address this challenge, we created the Mammalian Adipose Tissue Knowledge Portal (MATKP; matkp.org), an open community portal for multi-species mammalian adipose biology. MATKP is accessible to diverse users and aggregates data for adipose tissues and cell types, the genes active within them, and their relationship to human disease.Methods: We integrated expert-curated datasets, with an initial focus on transcriptomics. Reproducible analysis pipelines enable cross-dataset standardization. Key offerings include differential expression analysis of bulk RNA sequencing (RNA-seq) data and cell clustering, marker gene identification, and pseudobulk differential expression analysis of single nucleus RNA-seq (snRNA-seq) data.Results: Within MATKP, users can find, filter, or download bulk and snRNA-seq datasets from human and mouse adipose tissues. Cell types, tissue depots, and metadata fields are harmonized across datasets using standard ontologies. From each dataset, users can obtain analyzed results and visualizations. For snRNA-seq datasets, the single cell browser integrates interactive plots of cell type clusters, gene expression, cell type abundance, and marker gene expression. Users can query by gene name, compare gene expression, and filter by cell type or metadata field. Differential gene expression analyses are available for both pseudobulk snRNA-seq and for bulk RNA-seq data.Finally, gene queries provide gene functions, ontologies, and pathways; links to external resources; and aggregated summaries of gene expression across adipose tissue cell types, species, and datasets.Conclusion: MATKP serves as an integrative community resource to accelerate research in diabetes and adipose biology. Future MATKP iterations will include other data types (e.g., proteomics, imaging) and species (e.g., nonhuman primates).
J. Jurgens: None. M. Emont: None. T. Nguyen: None. A. Shilin: None. P. Smadbeck: None. D. Jang: None. M. Brandes: None. J. Flannick: None. E. Rosen: Consultant; Foghorn Therapeutics. Advisory Panel; Source Bio. N. Burtt: None.
National Institute of Diabetes and Digestive and Kidney Diseases (RC2 DK116691)
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