Introduction and Objective: Older adults with obesity are at increased risk for metabolic dysfunction and type 2 diabetes. Aging and excess adiposity promote the accumulation of senescent cells in adipose tissue (AT), contributing to chronic inflammation and impaired adipocyte function. Lifestyle interventions including weight loss and exercise improve metabolic health in older adults with obesity; however, whether these interventions reduce cellular senescence in human AT remains poorly understood.Methods: We are conducting an ongoing study investigating AT senescence in older adults with obesity (≥65 years; BMI 30-39.9 kg/m²; mean age 68.4 ± 3.9 years; mean BMI 33.3 ± 2.7 kg/m²) participating in a 10-week lifestyle intervention consisting of one hour (30 minutes of both endurance and resistance) exercise sessions three times per week combined with hypocaloric nutritional counseling. Subcutaneous AT biopsies were obtained before and after the intervention to evaluate markers of cellular senescence. Senescence-associated β-galactosidase (SA-βgal) staining was quantified in paired AT samples from nine participants before and after the intervention.Results: We find that lifestyle intervention leads to a 36% decrease in SA-βgal-positive cells (P < 0.05, n = 9). In a subset of participants (n=3), immunohistochemical analysis reveals a 60% decrease in p16 (P < 0.0001, n = 3), whereas p21 expression remained unchanged.Conclusion: These preliminary findings suggest that lifestyle intervention may reduce senescent cell burden in adipose tissue from older adults with obesity. Ongoing analyses in the full study cohort will further determine the impact of lifestyle interventions on adipose tissue senescence and metabolic health.
M. Kabir: None. G. Connolly: None. N. Zhang: None. D. Metin Armagan: None. A. Tazhibi: None. N. Mouallem: None. N. Todorova: None. J. Nie: None. L. Kao: None. K. Chen: None. S.E. Espinoza: None. A. Aslamy: None. N. Musi: None.
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